-------- Original Message --------
Subject: Re: Growing Arabidopsis in sand
Date: Thu, 03 May 2001 08:50:13 +0100
=46rom: Jim Tokuhisa <tokuhisa at ice.mpg.de>
Reply-To: tokuhisa at ice.mpg.de
To: Wendy Allan <wallan at uoguelph.ca>
References: <3AEEFB26.EFA193B8 at uoguelph.ca>
To continue yesterday's message:
An additional stress may be nutrients, too much or an imbalance. We
been using 0.1X Murashige & Skoog with vitamins and occasionally a 1X.
you want to stick with Hoagland's, you may want to use a diluted
perhaps include some N as ammonium. I chose M&S based on simplicity,
commercial availability, and multiple and repeated successful use with
Our growth conditions currently are to germinate Arabidopsis on soil
short-day, non flower-inductive conditions. Transplant 2 to 3-week-old
plants (4 to 6-leaf stage), with most soil removed, to plastic pots (2-3
6x6x10 cm^3 pots) containing Seramis wetted with 0.1X M&S. The pots are
trays with humidity domes and in a growth chamber with light intensities
soil level of about 70 umoles/m^2/sec. The domes are rather close to
lights--20 cm or so, but that is what we have to live with. Tray
temperatures are between 24-30 degrees C (30 is a bit high for
Arabidopsis). If the humidity is saturating, we leave the domes
off. We water as needed which is about once a week. When the plants
to grow like Topsy, we occasionally will water with 1X M&S.
My search for these growth conditions was based on what some folks have
to research nodules on leguminous roots. I also was inspired by the
soilless media used in hydroponic culture of house ornamentals--a
means of growing house plants particularly in Europe.
We have kept plants growing to seed set.
WEB sites to look at:
porous ceramic (we have not tried but it sounds promising if porous clay
Perlite: it works well, but is worse than soil for binding roots
Please keep me posted on your successes with this project. I am very
interested in any other techniques that work.
Jim Tokuhisa, Ph.D.
Max Planck Institut f=FCr Chemische =D6kologie
E-mail: tokuhisa at ice.mpg.de