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[Fwd: re: Growing Arabidopsis in sand]

Nobody nobody at hgmp.mrc.ac.uk
Thu May 3 09:03:31 EST 2001



-------- Original Message --------
Subject: re: Growing Arabidopsis in sand
Date: Thu, 3 May 2001 14:30:06 +0100
=46rom: <wunderwald at gsf.de> (Dr Ulrike Wunderwald)
Reply-To: <wunderwald at gsf.de>
To: <wallan at uoguelph.ca>


>To: arab-gen at net.bio.net
>Newsgroups: bionet.genome.arabidopsis
>From: wallan at uoguelph.ca (Wendy Allan)
>Subject: Growing Arabidopsis in sand
>Date: 1 May 2001 20:10:10 +0100
>Organization: Plant Agriculture Department
>Sender: owner-arab-gen at hgmp.mrc.ac.uk
>Precedence: bulk
>
>

Hallo Wendy,

seeds were sowed on (sterilized) quartz sand (1...2.5 mm) in pots (about
4.5
x 4.5 x 4.5 cm) soaked with water and vernalized at 4 =B0C under a plastic
wrap
for 2 days. The plastic wrap was removed 6 days after transfer to the
greenhouse. The light regime: 16 h light and 8 h darkness . The plants
were
fertilized with half concentrated sugar free Murashigge Skoog basal
medium pH
5.8. The water level should be about 2 cm. Take care that the water is
soaked
up to the surface. No algae did grow under these conditions.

The growth is relatively slowly in comparison to soil grown plants.
However,
after 4 weeks growth you get  reasonable plants. They can be grown for
about
8 weeks but I did not investigate the longest possible growing time.

Notice, only half of the germinated plants will survive.

It is necessary to use the right size of sand. Fine sand is useless for
this
purpose.

If you have more questions concerning this point, do not hesitate to ask
me.

Ulrike

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