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Arabidopsis oligonucleotide arrays

Imran Siddiqi imran at ccmb.ap.nic.in
Sat Mar 31 08:05:36 EST 2001

It would be really valuable to have access to such arrays at a reasonable
cost. At $100 a slide I would anticipate using approximately 50 slides
over the next 3-5 years. It would also be very nice if tester slides
comprising a small subset of genes could also be made available at a lower
cost for standardizing/calibrating/troubleshooting.

Imran Siddiqi

On 15 Mar 2001, Ellen Wisman wrote:

>  The Arabidopsis functional genomics consortium (AFGC:
>  http://afgc.stanford.edu) is applying for a renewal to develop a full genome
>  ~70-mer oligonucleotide array that could be used to measure gene expression
>  of almost all the genes of Arabidopsis.  Such oligonucleotide-based arrays
>  will provide higher gene specificity than the current PCR generated arrays
>  and thus greatly minimize cross hybridization between gene family members.
>  We anticipate that the data generated by the community using a standard
>  ~25,500 gene array will prove to be an important comparative resource for
>  expression studies.  Our goal would be to print up to 10,000 full genome
>  arrays over then next 3-5 years and distribute these to the community for
>  approximately $100/slide to use at their home institution. These arrays can
>  be read with all standard cDNA microarray readers and do not require
>  purchase of a specialized  workstation.  In addition to providing arrays
>  directly to the community, the current, full service microarray facility
>  would be continued at MSU with the new arrays for those who need or prefer
>  to use the facility for their experiments.  We believe this higher array
>  volume and lower cost will encourage wide use, and multiple biological
>  replications and therefore more reliable data will emerge.  It would help us
>  tremendously to know what the demand of the community for such microarrays
>  would be. Please let us know how many slides you might purchase and utilize
>  over the next 3-5 years in your research.  Please also note we do not
>  envision these arrays as a replacement for the Affymetrix arrays as both
>  have advantages and would find use in the community.
>  Ellen Wisman, Dean DellaPenna, John Ohlrogge
>  Ellen Wisman
>  Research Assistant Professor
>  MSU-DOE Plant Research Laboratory
>  Michigan State University
>  E. Lansing, MI 48824-1312
>  wismanp at pilot.msu.edu
>  (P) 517-353-4887
>  (F) 517-355-9298
>  http://www.prl.msu.edu/wisman/
>  ---


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