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purify RNA from proteoglycans and polysaccharides?

ZAG h225gast at edv2.boku.ac.at
Wed Mar 21 02:51:14 EST 2001

I prepared total RNA from plants (A. t.) grown on soil using Trizol
and have large amounts of contaminations of substances stated in a
for TRIFAST (from peqlab, similar to Trizol) could well be proteoglycans

and polysaccarides copurifying with RNA and having similar UV
They say in the manual, that a further purification with Optipure
as well) eliminates these problems. Does anybody have experiences with
these kind of problems who can provide help (experience with Optipure or

similar stuff, home-made protocols etc...).

thanks in advance,

Harald F. Sigmund

(to reply directly, please remove the dog: 
hsigmund at edv2.barkbarkbark.boku.ac.at)


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