the protein of our our interest is only slightly larger than rubisco. In
our western blots with cytoplasmic protein extracts the signal of our
protein of interest is sometimes overlaid by (the highly abundant)
rubisco, a problem which is not observed with nuclear extracts. Is there
a quick and simple way to get rid of rubisco in cytoplasmic extracts ?
Any comments and suggestions are greatly appreciated.
Ralf R. Weigel
Albrecht von Haller Institute of Plant Sciences
General and Developmental Plant Physiology
Untere Karspuele 2