I have an inquiry about antibiotic selection in Agrobacterium.
I have inherited a strain of agro that seems to not be sensitive to
streptomycin (50mg/ml) on YEP plates. Is this lack of selection due to:
1) too low of a streptomycin concentration?
2) the agro being contamination with a plasmid that has a streptomycin
3) streptomycin not being a selective agent in Agro? and is so, should I
be using Spectinomycin for my Agro selection.
Any insight would be appreciated.