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Non-radioactive Northern protocols

Nobody nobody at hgmp.mrc.ac.uk
Wed Apr 4 03:47:00 EST 2001

Dear all,

We are currently trying to use the digoxigenin system from Roche (previously
Boehringer) for Northern blots using RNA from several Arabidopsis tissues.
We are not getting signals, even with controls such as actin. Our probes
(DNA) are labeled by PCR, and we have used both double-stranded and
single-stranded. I would greatly appreciate help from anyone willing to
share his/her experience with this or other non-radioactive detection
systems in Arabidopsis Northerns.

Rafael R. Ariza, PhD
Departamento de Gen=E9tica
=46acultad de Ciencias
Avda. San Alberto Magno s/n
Phone: (34) 957 761 154
=46AX: (34) 957 761 297
e-mail: ge1roarr at uco.es


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