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Binary vector directly transform into Agro.

Dr. Hiranya S. Roychowdhury hroychow at nmsu.edu
Tue Nov 21 20:28:28 EST 2000


An old protocol utilized multiple freeze-thaw cycles on a plasmid-agro
suspension (I think cells were made comp with CaCl2). Never tried it
myself, but a very experienced colleague regularly used to he says, and
does sometimes now. The lab is pretty much settled on triparentals these days.

The other, much , much better (almost foolproof) method is, of course,
electroporation.

At 07:41 PM 11/21/00 -0000, Wendy Jin wrote:
>Hi all,
>
>Has anybody transformed binary vector into Agro. directly with going
>through E. coli?
>
>Thanks
>Wendy
>
>
>---
>
>
>
>
Dr. Hiranya Sankar Roychowdhury
College Asst. Prof.
Molecular Biology,
Dept. of Chemistry & Biochemistry
Box 30001 - 3MLS
New Mexico State University
Las Cruces, NM 88003

Lab: (505) 646 4722
Office: (505) 646 8256
hroychow at nmsu.edu


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