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RNAi questions

Jon Monroe monroejd at jmu.edu
Wed Nov 1 14:00:15 EST 2000

<x-flowed>Hi all,

We're in the process of making RNAi plasmids for our genes of
interest and have some questions:

1. does the orientation of the sense/antisense fragments matter?
	 <---xxxx--->  vs.  --->xxxx<---

2. does the size of the linker (xxxx) matter?

3. What should the linker be?  GUS has been used (Chuang and
Meyerowitz, 2000, PNAS 97:4985 PubMed #10781109) but could it be from
the gene of interest in either orientation?

Thanks for your help.

Jon Monroe

   Jonathan D. Monroe           	    Associate Professor
   Department of Biology, MSC 7801   office: 540-568-6649
   James Madison University	      fax: 540-568-3333
   Harrisonburg, VA 22807   	email: monroejd at jmu.edu



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