IUBio Biosequences .. Software .. Molbio soft .. Network News .. FTP

SEM of root hairs

Nicolas Baumberger nbaumber at botinst.unizh.ch
Wed Mar 15 07:55:21 EST 2000

Dear netters,
I would like to make scanning electron microscope pictures of
Arabidopsis root hairs grown on vertical MS plate. Unfortunately so far
I was never able to preserve the fine and delicate structure of the root
hairs. The main difficulty is that a loss of humidity of the air during
manipulation destroys the hairs in a matter of seconds. We tried to
freeze them in liquid nitrogen slush and do the observation in a SEM  at
low temperature but the short time needed (15-30 seconds) to transfer
the root in the freezing device was already too long for most of the
root hairs. In addition we had a lot of ice crystals at the surface of
the specimen which was difficult to sublime. We also tried to fix them 2
h with OsO4 vapors in a humid chamber followed by dehydration in acetone
but this too destroyed the hairs. I am desperatly looking for any advice
and trick  to make SEM observation of delicate structure like root
Thanks for any answer.

Nicolas Baumberger
Institute of Plant Biology, University of Zürich
Zollikerstr.107, CH- 8008 ZURICH, Switzerland
Tel: 41-1-6348232
Fax: 41-1-6348257
Email: nbaumber at botinst.unizh.ch


More information about the Arab-gen mailing list

Send comments to us at biosci-help [At] net.bio.net