This is how I did it:
Prepare Callus Inducing Medium (CIM) in Petri dishes:
pH adjusted to 5.7-5.8 with KOH
0.2 mg/l kinetin
1 mg/l 2,4-D
Prepare AM medium, also in Petri dishes:
0.5x MS salts
pH to 5.7-5.8 with KOH
Germinate sterilized Arabidopsis seed on AM medium, in the light.
After 10 days you should have seedlings with expanded cotyledons and
the first pair of true leaves just starting to show. Excise leaf
and/or hypocotyl pieces (you should do this under water in a Petri
dish, otherwise the seedlings dry out before you are finished) and
lay them on solidified AM medium. Place them in light and callus
growth should start soon.
If you need more details, please ask - I cannot know what your
previous experience is with this type of work.
Institute of Plant Biology
Biological Research Center
H-6701 Szeged, P.O.Box 521.
P.S. Your e-mail address doesn't seem to accept messages sent using
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