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A.thaliana plants dying after transformation procedure

zarir zarir at purdue.edu
Mon Jul 31 15:53:06 EST 2000

hi all,
i am trying to transform arabidopsis plants with my promoter constructs
in Agrobacterium tumefaciens GV3101 using the protocol on andrew bent's
website. The first time i used the recommended 0.05% Silwet L-77 and my
plants all died. The second time my plants died inspite of lowering the
conc. of the silwet to 0.03%. Any suggestions to what i can change in
the protocol from people with similar experience.

N-304 Beadle Centre
University of Nebraska-Lincoln
Lincoln, NE 68588-0665
Phone:- 402-472-6998(Lab)
Fax  :- 402-472-3139
Email:- zarir at purdue.edu
         nematode at expert.cc.purdue.edu


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