Another method is to grow the bacteria with sucrose as the only carbon
source, Agrobacterium can use sucrose but E coli cannot; Agrobacterium can
grow at 37C but cannot maintain its plasmids at that temperature so growth
with antibiotics at 37C could distinguish E coli from Agrobacterium.
Joel A. Kreps
Senior Staff Scientist, Plant Health
Novartis Agricultural Discovery Institute, Inc.
3115 Merryfield Row, Room 269
San Diego, CA 92121-1125 USA
joel.kreps at nadii.novartis.com
Dear Immo Junghaertchen,
E coli makes a single colony just for over night,
but Agrobacterium takes 2-3 days to make it.
Or Agrobacterium can not grow at 37C.
>I suspect E coli contamination in agrobacterium culture. Does anyone
>know of a quick way to distinguish between them? Under the microscope,
>both look like motile, gram negative rods of similar size!
>nr7654 at csc.albany.edu>>>---