A method using benzyl chloride was publishes a couple of years back in NAR.
I do not have the ref. handy, but a search w/ those two terms should be
sufficient. It works quite nicely for small amounts of tissue and yields
digestible and amplifiable DNA. However, it is a nasty compound and has to
be fresh to obtain good quality DNA. I suspect that old (>3mo) benzyl
chloride modifies certain bases. It is itself oxidized upon storage (hence
the need to store under inert gas). Unfortunately, it is hard to obtain the
compound in small amounts in the US. Ideally, buying about 25 to 50mL at a
time would be really great.
At 04:47 AM 7/24/00 +0100, Nobody wrote:
>I am looking for a protocol that can be used to
>prepare DNA for Southern blot from a little amount of
>Arabidopsis leaves( about 30-50mg of plant tissue). It
>will be deeply appreciated if anybody can give me some
>information about that.
>Dept. of CBMG
>Univ. of Maryland
>College Park, MD 20742
>Do You Yahoo!?
>Get Yahoo! Mail =F1 Free email you can access from anywhere!
>>>>Dr. Hiranya Sankar Roychowdhury
Dept. of Molecular Biology
PO Box 30001 - 3MLS
New Mexico State University
Las Cruces, NM 88003
Lab: (505) 646 4722
Office: (505) 646 8256
hroychow at nmsu.edu