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ProteinASepharose Purification of Antibodies

Jens Lohrmann lohrmann at sun2.ruf.uni-freiburg.de
Wed Sep 29 10:22:15 EST 1999

Dear Netters!

Just a short question:
Can you please send me or quote an easy and reliable protocol for
proteinAsepharose purification of antibodies? Especially I'm wondering
if there are alternatives to the acidic elution or how long you've got
to  incubate the sepharose/antibody with the acid.

Thanks a lot!

Jens Lohrmann

lohrmann at sun2.ruf.uni-freiburg.de


Schänzlestr. 1
Institut für Biologie II / Botanik
79104 Freiburg
Fon: 0761/203-2619
Fax: 0761/203-2612

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