most commertial 2-hybrid systems have AD and BD domains constructed at
N-terminal and only allow bait protein to be fused C-terminal to the AD or
BD domain, does anyone know if there is a system that allow my favorite
coding sequences to be constructed N-terminal to the AD or BD domain. Any
information will be greatly appreciated.
Assistant Research Fellow
Institute of Botany,
(Tel) 011-886-2-2782-3050-ext422 or 011-886-2-2789-9590 ext 422