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Postdoc available: Nuclear-Cytoplasmic Interactions in Maize

Roger P. Wise rpwise at iastate.edu
Mon May 24 19:31:21 EST 1999

Molecular Genetics of Nuclear-Cytoplasmic Interactions in Maize

Postdoctoral Research Associate available August 1.  The candidate will
be responsible for the isolation and functional characterization of
nuclear (Rf)  genes that mediate mitochondrial RNA processing and the
restoration of male fertility in T-cytoplasm maize.  Male sterility in
T-cytoplasm maize is due to the presence of a novel mitochondrial gene,
T-urf13.  Full (or partial) fertility restoration of T-cytoplasm is
mediated by one of three (Rf1, Rf8, or Rf*) nuclear restorers, in
combination with the Rf2 restorer.  Rf2 encodes a protein highly
similar to mitochondrial aldehyde dehydrogenases; Rf1-, Rf8-,and Rf*
each mediate discrete T-urf13 transcript processing events.  This
USDA/NRI-funded project is to physically characterize the genomic
region spanning Rf1, use AFLP-transposon display to clone and
characterize additional mutant alleles of Rf1, and to test the
functionality of candidate Rf1 sequences via  our newly developed
T-cytoplasm, transformation system.  The candidate will have access to
a wide array of rf1-m mutants and recombinant populations as well as
BAC library resources and high-throughput sequencing capability.

Literature references:

Wise, RP, CL Dill, and PS Schnable. 1996.  Mutator-induced mutations of
the rf1 nuclear fertility restorer of T-cytoplasm maize alter the
accumulation of T-urf13 mitochondrial transcripts. Genetics

Dill, CL, RP Wise, and PS Schnable. 1997.  Rf8 and Rf* mediate unique
T-urf13-transcript accumulation, revealing a conserved motif associated
with RNA processing and restoration of pollen fertility in T-cytoplasm
maize. Genetics 147:1367-1379.

Wise, RP, CR Bronson, PS Schnable, and HT Horner. 1998. The genetics,
pathology, and molecular biology of T-cytoplasm male sterility in
maize.  Advances in Agronomy 65: 79-130.


Ph.D. in genetics, molecular biology, plant physiology, or related
field.  Experience in manipulation and cloning of large DNA fragments,
gene mapping, computational analysis, and/or cereal transformation is
desirable.  Initial appointment is for two years; salary starts at
$33,026 per year + benefits.  Some limitations on citizenship may apply
(see below).  Highly motivated individuals should send curriculum
vitae, reprints, and references (name, e-mail, address, phone, and fax
no.) to:

Dr. Roger Wise, USDA-ARS

Department of Plant Pathology

351 Bessey Hall

Iowa State University

Ames, IA, 50011

Telephone:   (515) 294-9756

Fax: 	    (515) 294-9420

E-mail:	    rpwise at iastate.edu



>About Iowa State University

Ames (http://www.ames.ia.us/) is a small city of 50,000 people, about
half of who are students
 Affordable housing is available as well as a first-rate public
transportation system.  It is also easy to get around town by bicycle.
The city and the University  (http://www.iastate.edu/) have numerous
parks and recreation facilities, and the Iowa State Center, home of
Hilton Coliseum, C.Y. Stephens Auditorium and Fisher Theater, hosts
numerous music concerts, live performances, and athletic events
throughout the year.

[Citizenship limitations on this position (funded through a
NRI-competitive grant to USDA-ARS), requires that it be filled by 1)
citizens of the United States, 2) a citizen of a country which is a
member of a defense treaty, eg., NATO, SEATO, RIO treaty or 3) by
citizens of countries which the U.S. congress has specifically exempted
from the restricting legislation.  These countries include: Argentina,
Australia, Bahamas, Belgium, Bolivia, Brazil, Canada, Chile, Columbia,
Costa Rica, Cuba, Denmark, Dominican Republic, Ecuador, El Salvador,
France, Germany, Greece, Guatemala, Haiti, Honduras, Iceland, Italy,
Japan, South Korea, Luxembourg, Mexico, Netherlands, New Zealand,
Nicaragua, Norway, Panama, Paraguay, Peru, Philippines, Portugal,
Spain, Thailand, Tobago, Trinidad, Turkey, United Kingdom, United
States, Uruguay, Venezuela, Ireland, Israel, (South Vietnamese,
Cambodian, and Laotian refuges paroled into the U.S. after January 1,
1975), (Aliens from Cuba, Poland, South Vietnam, countries of the
former Soviet Union, Estonia, Latvia, and Lithuania admitted to the
U.S. for permanent residence), (Natives of Guam, Puerto Rico, and the
Northern Mariana Islands), and (Nationals of the Peoples Republic of
China that were in the U.S. on or before June 5, 1989 up until April
11, 1990, and qualify under the Chinese student protection act of


Roger Wise, USDA-ARS

Department of Plant Pathology

409 Bessey Hall

Iowa State University

Ames, IA

50011-1020  USA

Phone:  515-294-9756

Fax:    515-294-9420

E-mail: rpwise at iastate.edu


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