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Postdoctoral position

Leon Kochian lvk1 at cornell.edu
Tue Jan 27 16:11:14 EST 1998

                                ARS POSTDOCTORAL RESEARCH ASSOCIATE
                                        CORNELL UNIVERSITY

Funding is available immediately for an ARS postdoctoral associate to
investigate the molecular biology and physiology of heavy metal transport
and tolerance in the heavy metal (zinc and cadmium) hyperaccumulator plant
species, Thlaspi caerulescens, in order to better understand these
processes in relation to the use of plants to remediate heavy metal
contaminated soils. We have recently cloned a Zn transport cDNA from
Thlaspi caerulescens and the research will address the molecular and
physiological characterization of this cDNA and the transport protein it
encodes. The molecular research will focus on 1) examining the regulation
of gene expression in response to changes in plant mineral status; 2)
determining the cellular location of the transport protein; 3) evaluating
the role of the transporter in plant heavy metal accumulation through the
use of transgenic Arabidopsis seedlings; and 4) cloning other heavy metal
transport genes from Thlaspi caerulescens and related non-accumulator
species. Physiological investigations will involve use of radiotracer flux
techniques to characterize the Zn/Cd transport properties of this cDNA
expressed in heterologous systems (e.g. yeast). Applicants should have a
Ph.D. in Plant Molecular Biology, as well as research experience in
recombinant DNA techniques, including in situ  hybridization,
immunolocalization, and plant transformation. A basic understanding of
plant mineral ion transport processes is desirable. Salary for this
position is $34,839/year. Applicants should mail a CV, statement of
interest and names of three references to Dr. Leon Kochian, U.S. Plant,
Soil and Nutrition Laboratory, Cornell University, Tower Rd, Ithaca, NY 14853.

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