Recently I did western blots to detect the protein I am working on in the
Arabidopsis. I got a problem in which the anti-mouse IgG with peroxidase
conjugate from Sigma recognized the Arabidopsis crude proteins and gave two
or three nonspecfic bands even in 10,000 dilution. Does anybody out have the
same problem? How can I get rid of these nonspecific bindings? Where can I get
the secondary antibody which does not cross-react with Arabidopsis protein?
Thanks very much.
Department of Botany
University of Geogia