I do not know how my name got attached to your posting to the
Arabidopsis net, but I might have the answer.
The George Haughn has cloned a mutant Arabidopsis ALS gene that
confers resistance to the herbicide chlorsulfuron. Details are in:
1. Haughn and Somerville, Mol. Gen. Genet. (1986) 204-430-434.
2. Haughn et al., Mol. Gen. Genet. (1988) 211:266-271.
George has supply me with a plasmid containing this gene. I'm sure he
is willing to supply it to you. He can be contacted at the U of British
Columbia at: Haughn at unixg.ubc.ca
Chlorsulfuron can be purchased from Chem Services Inc., P.O. Box
3180, West Chester, PA 19381. 100 mg is about $50.00 U.S.
Purrington and Bergelson (Genetics, 145:807-814, 1997) has placed
this gene into a pBIN19 derivative, and used it to transform Arabidopsis.
This might me a useful vector for you. Good luck.
Stokes S. Baker, Ph.D.
Assistant Professor of Biology
University of Detroit Mercy
P.O. Box 19900
Detroit, MI 48219-0900
Phone: (313) 993-1142
FAX: (313) 993-1139
E-mail: bakerss at udmercy.edu or au186 at detroit.freenet.org