we are working with a protein, which has several putative myroystilation
sites. We suspect that these sites function as a membrane anchor, because
we were not able to solubilize the protein with a number of detergents we
have tested so far. I am wondering whether somebody knows how to cleave such
sites. We found in a paper that an enzyme called polymyxin acylase is able
to cleave myroystilation sites, but we could not find a source for the
enzyme. Does anybody know a source for this enzyme or is willing to share
with us small amounts for initial experiments? Any other suggestions for
solubilization are also welcome.
Thanks in advance.
60054 Frankfurt, Germany
Ludwig-Mueller at em.uni-frankfurt.de