IUBio Biosequences .. Software .. Molbio soft .. Network News .. FTP

DNA extraction from root culture

Maurizio Chiurazzi chiurazzi at IIGBNA.IIGB.NA.CNR.IT
Mon Mar 18 05:57:37 EST 1996


Dear netters,
I'having some trouble with DNA extraction from root culture. I'm using a
lysis buffer 7M urea and 1% Lauroylsarcosine that works pretty well with
leaves DNA extraction. The DNA yield is not so good, around 15 microgram
per 200 mg of tissue and it looks very viscous (polysaccarides?) and do not
give complete digestion.
Any advices?
thanks
Maurizio





More information about the Arab-gen mailing list

Send comments to us at biosci-help [At] net.bio.net