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Cosmid vectors

John M. McDowell mcdowell at email.unc.edu
Thu Mar 14 22:28:43 EST 1996

> The only trouble is that the Agrobacterium strain normally used, GV3101,
> mutates to Tc resistance at high frequency.
We've been able to circumvent this problem by using Kan50 to select for
transformants with pCLD04541.  Apparently the 35s promoter is active
enough in Agro. for this to work.

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