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PhD studentship

STEPHEN A ROLFE S.Rolfe at sheffield.ac.uk
Wed Mar 13 08:23:20 EST 1996

I would be most grateful if this position could be brought to the attention of any 
suitable candidates.

MAFF-funded studentship starting October 1996
Contact either:
Drs Julie Scholes and Stephen Rolfe
Robert Hill Institute
Department of Animal and Plant Sciences
University of Sheffield
Tel: 0114 2824115 or 2824780
Fax: 0114 2760159
e-mail s.rolfe at sheffield.ac.uk
       j.scholes at sheffield.ac.uk
Infection of members of the Brassicacae with Plasmodiophora brassicae W. (clubroot) 
is of considerable agricultural importance resulting in the formation of a gall as a result 
of a re-initiation of cell division in the hypocotyl or root.  Gall formation is closely 
linked with alterations in plant growth regulators most probably as a result of an 
decrease in the auxin:cytokinin ratio; elevated auxin levels are associated with 
resistance.  This gall acts as a strong sink for carbohydrate reducing yield and can also 
damage the vascular system of the plant.

The project aims to identify genes expressed in response to clubroot infection and use 
their promoters as a basis for engineering resistance.  Initial studies will use the model 
system Arabidopsis thaliana as infection of this plant with clubroot is well 
characterised at the physiological level and it is amenable to the molecular biological 
strategies to be employed.  As a member of the Brassica results will be directly 
transferable to agriculturally important crops.

A wide variety of physiological, biochemical and molecular techniques will be applied 
in this project and full training will be provided.  The studentship is for 3 years 
commencing on 1st October, 1996.  Candidates must possess an upper second class 
honours degree (or better).  The standard MAFF student stipend will be paid which is 
currently 5638 pounds sterling per annum with additional allowances 
for dependants.

Department of Animal and Plant Sciences
University of Sheffield

Tel: (+44) 1142 824115
Fax: (+44) 1142 760159
e-mail: s.rolfe at sheffield.ac.uk

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