The 1996 Cold Spring Harbor Arabidopsis Molecular Genetics Course will be
held from 30 June to 20 July. A description is included below. The deadline
for applications is 15 March. Please note that a limited number of partial
scholarships are available thanks to funding from the National Science
For more information please contact the Meetings and Courses office at CSHL:
email: meetings at cshl.org
www http://www.cshl.org/ (applications available online)
Xing-Wang Deng, Department of Biology, Yale University
Robert Last, Boyce Thompson Institute for Plant Research at Cornell University
Daphne Preuss, Department of Molecular Genetics and Cell Biology,
Univeristy of Chicago
This course provides an intensive overview of topics in plant growth and
development, focusing on molecular genetic approaches to understanding plant
biology. It emphasizes recent results from Arabidopsis thaliana and other
model plants and provides an introduction to current methods used in
Arabidopsis research. The course also demonstrates the use of microbial
systems in plant research, including Agrobacterium, E. coli, and
S. cerevisiae. It is designed for scientists with experience in
molecular techniques or in plant biology who wish to work with Arabidopsis.
The course consists of a vigorous lecture series, a hands-on laboratory, and
informal discussions. Speakers will provide both an in-depth discussion of
their own work and a review of their specialty.
Discussions of important topics in plant research will be presented by the
instructors and by invited speakers. These seminars will review plant
anatomy; plant development (including development of flowers, roots,
meristems, embryos and the epidermis); perception of light and
photomorphogenesis; responses to pathogens and to DNA damage; synthesis and
function of secondary metabolites and hormones; nitrogen assimilation;
unique aspects of plant cell biology (including the plant cytoskeleton,
cell wall, and
chloroplasts); the importance of transposons and Agrobacterium for
manipulating plant genomes and current approaches to genome analysis.
Invited speakers include: D. Beach, P. Benfey, D. Bush, A. Cashmore, C.
Chapple, M. Cherry, C. Falco, J. Glazebrook, P. Hepler, D. Jofuku, B.
Keith, K. Kindle, A. Lloyd, R. Martienssen, R. Meagher, S. Poethig, R.
Sederoff, and G. Walker.
The laboratory sessions will provide an introduction to important techniques
currently used in Arabidopsis research. These include studies ofArabidopsis
development, mutant analysis, studies of epidermal features, in
situ detection of RNA, histochemical staining and immunolabeling of proteins,
transformation with Agrobacterium, transient gene expression in protoplasts,
expression of plant proteins in microorganisms, detection and analysis of
plant pathogens, and techniques commonly used in genetic and physical
Robert L. Last