This may be a niave question but I am new to using YACs :-
I plan to isolate left end probes from YACS from both the EG and EW libraries.
The method I have is straight forward but the first step involves digesting the
yeast DNA with XhoI, NdeI or XhoI + SalI. Does the choice of enzyme depend on
which library the YAC is from or what?
Thanks for the help