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End probes/Plasmid rescue

Carol Johnstone gbcb42 at udcf.gla.ac.uk
Fri Aug 30 06:09:46 EST 1996

This may be a niave question but I am new to using YACs :-

I plan to isolate left end probes from YACS from both the EG and EW libraries. 
The method I have is straight forward but the first step involves digesting the 
yeast DNA with XhoI, NdeI or XhoI + SalI. Does the choice of enzyme depend on 
which library the YAC is from or what?

Thanks for the help


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