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Problems with GUS batches

Melissa Melan melan at duq3.cc.duq.edu
Thu Aug 29 14:26:07 EST 1996

Hmmmm...*is* there a problem with X-Gluc from Rose?

I had problems with tissue staining a few months ago and assumed that 
it was due to my (and my students') lack of expertise and not the 

I had an advanced undergraduate lab produce transgenic tobacco with 
pBI121.  We assayed tissue with p-nitrophenyl-Gluc and also did tissue 
staining with X-Gluc.  We got enzyme activity values for 20:20 plants 
with p-nitrophenyl-Gluc but tissue staining for only 3:20 plants 
(roots only; no staining in _any_ leaves).  The tissue staining was 
done using both vacuum infiltration and Triton-X 100 in the stain 
mixture.  The tissue staining was not restricted to only those plants 
with high enzyme activity.  I was (and still am) at a loss to explain 
this.  If anyone has any viable explanations for getting consistent 
tissue staining please pass them along.  Thanks!

Melissa Melan
Department of Biological Sciences
Duquesne University
Pittsburgh, PA  15282
(412) 396-5565

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