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T3 Arabidopsis transgenics won't grow????

Sally stephenson at tpp.uq.edu.au
Tue Aug 27 09:40:26 EST 1996

I have been germinating transgenic Arabidopsis seedlings on a 3% 
Sucrose MSO medium (with 50 Micrograms/ml kanamycin for transgenic 
selection).  The T2 generations germinated OK after 2 days 
vernalisation. BUT, the T3 generation seeds obtained from self 
fertilisation of the T2 plants won't germinate under the same 
conditions (it appears they won't germinate at all).

Has anyone experienced a similar problem?  Any advice would be greatly 


Andrew Morgan
CRCTPP, University of Qld.

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