I have been germinating transgenic Arabidopsis seedlings on a 3%
Sucrose MSO medium (with 50 Micrograms/ml kanamycin for transgenic
selection). The T2 generations germinated OK after 2 days
vernalisation. BUT, the T3 generation seeds obtained from self
fertilisation of the T2 plants won't germinate under the same
conditions (it appears they won't germinate at all).
Has anyone experienced a similar problem? Any advice would be greatly
CRCTPP, University of Qld.