It's not easy, but check out C.R. Somerville et al. in Plant Science
Letters 21, 89-96 (1981). I'm not aware of any major alterations since.
Anders B. Jensen wrote:
>> I need a good protocol for isolation of chloroplasts from Arabidopsis,
> Is there someone who has a good protocol?
> I will be happy to get a copy.
>> Tine Hoff
>hoff at biobase.dk