Hi all --
Someone here is trying to do immunolocalization of a membrane-bound
protein with an affinity purified antibody, and is having lots of problems
with nonspecific binding of her primary antibody to starch granules. So
far, she's tried lots of different blocking conditions (gelatine, serum,
BSA, milk), and several different fixation conditions, embedded in LR
White and in cryosections, but hasn't seen much improvement. Does anyone
have suggestions on other techniques she could try to reduce the
background? Thanks very much!
Please send email to bmhong at scripps.edu.