Does anybody have the sequence data for the YAC-vector pYAC3 used to
construct the EW-YAC library. The only available YAC-vector sequence
is pYAC4 which is different from pYAC3!!We have difficulties to cut
out the A.th. DNA insert after a XhoI plasmid rescue from the EW-YACs.
Some predicted restriction sites seem not to be there and some are
there which should not!Any sequence datas and/or restriction sites
would be of great help.
Thanks in advance