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X-gluc problems

Michael R. Thompson mthompson at pbi.nrc.ca
Thu Jan 26 18:28:17 EST 1995


I am attempting histochemical staining of salt-stressed Arabidopsis 
transformants, using x-gluc to detect GUS activity. Non-stressed plants 
develop low levels of indigo during staining; stressed plants produce some 
indigo in superficial cell layers and develop a red stain in underlying
cell layers. The latter slowly (over several days) turns blue during 
clearing/fixation using chlorallactophenol. Does anyone know if salts 
(in this case sodium and magnesium sulphates) inhibit oxidative dimerization
of the indoxyl cleavage product of the reaction? Or if there is perhaps a 
more mundane explanation?

Thanks in advance for any help. I can be reached at the above e-mail address.

Mike Thompson
Plant Biotechnology Institute
Saskatoon, Canada.



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