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DNA isolation

Koop Hans-Ulrich 208 koop at botanik.biologie.uni-muenchen.de
Fri Jan 20 12:57:45 EST 1995

We need to check hundreds of putative transformants (tobacco) for the
presence of the transgene. We want to use PCR and Southerns. Would anybody
share their favorite protocol for isolating DNA from many samples of callus
and/or shoots: at very low costs, quickly, reliably, without 'nasty' chemicals,
and yielding tons of high quality DNA? Thanks!!!!!!! Ulrich
koop at botanik.biologie.uni-muenchen.de   

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