Dear Arabidopsis researchers,
Has anyone used the GPA1 CAPS mapping primers that were purchased from
Research Genetics. I have tried them under the same protocol as indicated
in Konieczny and Ausebel's paper and have not gotten it to generate any PCR
product. Is this primer used under different MgCl2 concentration or
annealing temperature than those used for other CAPS primers.
Other CAPS primers from Research Genetics seem to work fine.