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in situ for viruses

FUENTES at PARGVA.AGR.CA FUENTES at PARGVA.AGR.CA
Thu Jan 5 11:40:57 EST 1995


I have been using immunocytochemical techniques to look at viral
localization in Arabidopsis embryos.  I would like to use in situ
hybridization to look for viral RNA in the developing flowers and
embryos.  Could anyone send me a protocol, with embedding and
fixing procedures included, for in situ hybridization on thick
sections?  I have some references but would appreciate a little
more detail.  Thank you very much.
Ana Lucia Fuentes
Email: fuentes at pargva.agr.ca   



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