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New RFLP Map

ARABIDOPSIS arabidopsis.arabidopsis at afrc.ac.uk
Tue Sep 27 06:37:18 EST 1994


    An updated RFLP map, produced on the Landsberg erecta x 
Columbia Recombinant Inbred lines, is now available. The map 
has also been submitted to AAtDB.

    The map gives the position of 379 RFLP markers. There are a 
few changes in the order of previously mapped markers compared 
with the last map (June 1994). This is partly as a result of the 
addition of 43 new markers, but also in the random way in which 
"Mapmaker" selects markers to produce an order. Also we must 
apologise for a few mistakes in marker names on the previous map 
and hope this has not caused too much confusion!

    A drawn map has not in fact been produced, instead a list of 
the marker orders and the cM distances to the next marker are 
shown. The maps for Chromosomes 1, 2, 3 and 5 have been 
generated completely using "Mapmaker". For Chromosome 4 we have 
used the physical map data, from Renate Schmidt and her 
co-workers, to create the framework for the map around which the 
new markers have been positioned.

    The accuracy of the map is determined by several factors, 
the most important of which is the quality of the segregation 
data submitted. Secondly, the use of 100 lines means that 
recombination events between closely linked markers are becoming 
limiting.

    To obtain either a hard copy or an e-mailed copy of the map 
please contact Mary Rayward-Smith at ARABIDOPSIS at BBSRC.AC.UK
Any enquiries about markers on the map should also be directed 
to ARABIDOPSIS.

    The next new map will be produced in time for the Plant 
Genome III meeting in January. Please send your segregation data 
to LISTER at BBSRC.AC.UK

Clare Lister and Caroline Dean 



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