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Conflicting opinions regarding NBT product solubility

markw at UNIXG.UBC.CA markw at UNIXG.UBC.CA
Mon Sep 26 13:39:40 EST 1994

Dear Arabidopsis community, 

        About 1 week ago a summary of in situ protocols was posted to the 
group by Dr. Yi-Fang Tsay.  Among these were observatons about the 
solubility of the NBT product in Ethanol or Xylene.  My experience (from 
personal disasters!) is that the NBT product is soluble in Xylenes, but 
hardly soluble in Ethanol. This is contradictory to the opinions of other 
respondants who claim solubility in Ethanol, but NOT Xylene/Histoclear (see 
Dr. David Jacksons repsponse).

Is there a general consensus about which of these (or both) is correct?  Is 
there any reason why this might be true in one case but not in others?  
Please share your experiences with the group, as this seems a fairly 
important issue...


Mark Wilkinson
markw at unixg.ubc.ca

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