Dear Arabidopsis community,
About 1 week ago a summary of in situ protocols was posted to the
group by Dr. Yi-Fang Tsay. Among these were observatons about the
solubility of the NBT product in Ethanol or Xylene. My experience (from
personal disasters!) is that the NBT product is soluble in Xylenes, but
hardly soluble in Ethanol. This is contradictory to the opinions of other
respondants who claim solubility in Ethanol, but NOT Xylene/Histoclear (see
Dr. David Jacksons repsponse).
Is there a general consensus about which of these (or both) is correct? Is
there any reason why this might be true in one case but not in others?
Please share your experiences with the group, as this seems a fairly
markw at unixg.ubc.ca