Thanks to all who replied to my in-planta transformation technique question
(why is it necessary to use two agrobacterium inoculations?).
The upshot is: no one really seems to know why two inoculations are
necessary - it's just the protocol. Apparently, no one has tried the one
-inoculation control to see if the efficiency is lower. I suspect that
the second inoculation is the only one really necessary, but
it is hard to be sure on this.
The other consensus seemed to be that vacuum infiltration was a far
Thanks again, all.
University of Chicago
Department of Ecology and Evolution
irene at pondside.uchicago.edu