I am currently attempting to transform Nicotiana langsdorffii with
LBA4404, but without much success. So far I have generated one callus, from
about 40 leaf discs.
If anybody has transformed N. langsdorffii with A. tumefaciens, I
would be very greatful to know what strain of A. tumefaciens was used and how
you did it.
Univ of Otago