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Fidelity of polyadenylation in plants

Marty Sachs msachs at uiuc.edu
Fri Sep 16 00:01:13 EST 1994

In article <35a5ae$i1p at mark.ucdavis.edu>, csgasser at ucdavis.edu (Chuck
Gasser) wrote:

: Peter Hare asked about poly-adenylation sites in plants.
: For the more than ten different mRNAs for which I have sequenced
: multiple
: cDNA clones only one or two genes had consistent poly-A addition sites.
:  All
: of the others showed variability in the site of poly-A addition,
: varying in
: position by up to 50 bases.  These range from EPSP synthase in tomato
: to 
: some of the Arabidopsis cyclophilin genes.  I would be interested to
: know if
: there are any publications addressing this apparently common
: phenomenon.
: Chuck Gasser
: csgasser at ucdavis.edu

Here are a few....

Dean, C., S. Tamaki, P. Dunsmuir, M. Favreau, C. Katayama, and H.
Dooner.    mRNA transcripts of several plant genes are polyadenylated at
multiple sites in vitro. Nucleic Acids Res. 14:2229-2240  1986

Dhaese, P., H. De Greve, J.  Gielen, J. Seurinck, M. Van Montagu and J.
Schell       Identification of sequences involved in the polyadenylation
of higher plant nuclear transcripts using Agrobacterium T-DNA genes as
models EMBO J. 2: 419-426   1983

Hunt, A.G.     Identification and characterization of cryptic
polyadenlyation sites in the 3' region of a pea ribulose-1,5-bisphosphate
carboxylase small subunit gene  DNA 7: 329-336 1988

Hunt, A.G., N.M. Chu, J.T. Odell, F. Nagy and N.-H. Chua      Plant cells
do not properly recognize animal gene polyadenylation signals  Plant
Molecular Biology 8: 23-35 1987

Hunt, A.G. and M.H. MacDonald     Deletion analysis of the polyadenylation
signal of a pea rubulose-1,5-biphosphate carboxylase small-subunit gene 
Plant Molec. Biol.  13: 125-138  1989

Joshi, C.P.     Putative polyadenylation signals in nuclear genes of
higher plants: a compilation and analysis  Nucleic Acids Res. 15:
9627-9640 1987

Mogen, B., M.H. MacDonald, R. Graybosch, A.G. Hunt     Upstream sequences
other than AAUAAA are required for efficient messenger RNA 3' end
formation in plants Plant Cell 2: 1261-1272 1990

Sachs, M.M., E.S. Dennis, W.L. Gerlach, and W.J. Peacock      Two alleles
of maize Alcohol dehydrogenase 1 have 3' structural and poly(A) addition
polymorphisms Genetics 113: 449-467   1986

Sanfacon, H., P. Brodmann, T. Hohn       A dissection of the cauliflower
mosaic virus polyadenylation signal  Genes & Development.  5: 141-149 1991

Marty Sachs              
USDA/ARS & Agronomy/UIUC    msachs at ux1.cso.uiuc.edu [internet]
S108 Turner Hall            msachs at UIUCVMD [bitnet] 
1102 S. Goodwin Ave.        (217) 244-0864 [phone]
Urbana, IL  61801-4798      (217) 333-6064 [FAX]

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