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can I root regenerates in soil?

June I. Medford jim2 at PSU.EDU
Wed Sep 14 17:12:40 EST 1994


Kathy, We used to do this all the time and it works quite well.  It helps
to have a little bit of callus still attached to the shoot.  This way you
don't need to get a real great root and it will still set seed.  We used
rootene F--with Fungicide from the local discount store.  Dip the callus or
end of the shoot in the  power, shake off the excess and put in the soil.
When you put it in the soil, place a small hole in the soil, place the
plant in and push the soil carefully around it.  Then put the shoot in a
plastic bag (Consolidated Plastics, Ohio).  Fold the bag over at the top
and staple it.  Be careful to keep the time the plant is out in the
non-humid air to a min.  After 3-4 days or when growth is obvious, open the
stapled bag.  Then slowly over approx. 5-7 days ease the plant out by
progressively cutting the bag.  We have switched to more advanced methods
(e.g., in planta and whole plant) nowadays.  GOOD LUCK!


Dr. June I. Medford
Department of Biology
506 Wartik Laboratory
Pennsylvania State University
University Park, PA 16802 USA
Telephone:  (814)-863-6092
FAX: (814)-865-9131
EMAIL:  jim2 at psu.edu





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