We need to do some 5' RACE (or anchored) PCR with
Arabidopsis cDNAs. In some protocols G or C tailing
is used, in others, T or A tailing is used because of
the lower Tm.
We wondered if the relatively high AT content of
Arabidopsis might make it a bad idea to use T or A
tailing of 5' ends of cDNAs. Could the high internal
AT content cause spurious PCR priming?
Does anyone have suggestions about this?
Russell L. Malmberg
russell at dogwood.botany.uga.edu