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cloning & sequencing large PCR products

hayworth at WUSTLB.WUSTL.EDU hayworth at WUSTLB.WUSTL.EDU
Wed Oct 19 14:57:40 EST 1994


I'm would appreciate suggestions for how I might most easily clone and sequence
large (5000 bp) products.  The repetitive nature of the region I am studying
prevents me from be able to design sequencing primers which are internal to the
sequence.  Therefore, I need a system (kit) with which I can generate a series
of deletions from the initially cloned PCR product.  Are kits with these
features commercially available, and if so, which ones are recommended?  Are
there completely different methods for obtaining the entire sequencing of
large PCR products that I should consider?

Thanks for any help people can give me,

Doug Hayworth
Washington University
Hayworth at wustlb.wustl.edu




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