We are investigating a gene in Arabidopsis that is downregulated by light
(minimal detection after 8h light). We were wanting to fuse several portions
of the promoter to a reporter gene and assess their ability for light
responsiveness. The presumed problem with gus is the stability of the protein
(50h in protoplasts according to Jefferson 1987). Has anyone tried a similar
experiment using histochemical analysis of gus or do we need to assess the activity
of the promoter regions at the mRNA level? Or does anyone have a suggestion
of a different reporter gene that may be useful in this situation?
Thankyou for your help.
University of Auckland, New Zealand
email ledger at sbsu1.auckland.ac.nz