I have been growing transgenic Arabidopsis for some time now on MS
media solidified with Sigma's Phytagel. Occasionally, a few of my
seedlings will develop a 'vitreous' condition in which the leaves appear
translucent, sometimes almost to the point of transparency. This seems to
occur more frequently if the plants are over-crowded in their petri
dishes. Other than that, I have not been able to pin down the cause. One
person I spoke with suggested that the Phytagel I am using might be part
of the problem. He told me he had heard that Phytagel can cause problems
with Arabidopsis, although he couldn't give me any solid information.
I am doing a small study now to compare germination and growth of
my transgenic and wild-type seeds on Sigma Phytagel versus Gibco Phytagar.
But I was wondering if anyone knows anything about the cause of the
'vitreous' condition, and/or the compatibility of Arabidopsis and
Dept. of Molecular and Cellular Biology
University of California at Davis
(mwcrepeau at ucdavis.edu)