>>As a neophyte Arabidopsis grower I am having problems getting seed, that I
>>have grown myself, to germinate. I am working with Columbia. I harvested
>>pods when they were golden and dry. I thought that 1 or 2 weeks of
>>afterripening at room temperature would be enough but they still don't
>>germinate. I already know that they need a light-treatment to germinate.
>>>>Is there a higher or lower temperature treatment that I should try in order
>>to break this dormancy? I'm kind of in a rush.
>>>>Thanks in advance!
>>>>Dave Lowell Robinson
>>St. Olaf College
>>>>robinsod at stolaf.edu
To All Those Who Answered My Query:
The general consensus was that I should imbibe the seed and treat with 4 C
for about 3 days before trying to germinate. Others offered that a little
gibberellic acid, or that drying the seed with a desiccant, would also help
if the cold treatment didn't work.
As soon as you replied I put my dishes (sown on agar a few days before and
kept at room temperature) into my refrigerator for 3 days and sure
enough....now after only 8 hours at room temp I've got radicles emerging!
I (and my students trying to grow Arabidopsis) thank you!
Dave Lowell Robinson