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Joe Ecker jecker at ATGENOME.BIO.UPENN.EDU
Tue May 31 13:21:15 EST 1994

Dear Arabidopsis Researchers,

On June 8 and 9 the North American Arabidopsis Steering Committee-NAASC,
(current members are: Ausubel, Chory, Estelle, Ecker, Meinke and Nina Fedoroff
-substitute for Gloria Coruzzi, will meet at NSF headquarters along with 
members of the Multinational Steering Committee, genome experts and 
representative from the federal granting agencies to discuss issues related to
mapping and sequencing of the Arabidopsis genome.   As stated in "A Long-Range 
Plan for the Multinational Coordinated Arabidopsis thaliana Genome Research 
Project" (NSF document 90-80),  the mission of this project is to "identify 
all of the genes by any means and to determine the complete sequence of the 
Arabidopsis thaliana genome before the year 2000". 

The purpose of the NAASC workshop is to assess the progress in meeting our 
short and long term goals in the areas of mapping and sequencing of the 
Arabidopsis genome and make specific recommendations to direct future US 
efforts for the Multinational Coordinated Arabidopsis Genome Research Project.  
We will produce a short report on the needs for future genome-related efforts 
for the US federal funding agencies.

We want everyone to be included in this discussion and so we are soliciting 
your input into this report.  Please read over the following questions and the 
comment back either to the network or to me directly.  We shall consider all 
of your comments at the workshop.

Please read and respond to the following questions:

1. Do you think that we are ready to begin some level of directed genome 
sequencing in the US?  

2. How important is genome sequencing in terms of funding priorities (vs. 
placing cDNAs on the map, completion of the physical map, adding more 
PCR-based markers to the map, etc.)?

3. Who should support systematic genome sequencing if it is a big-$ effort?

4. What impact on Arabidopsis research will be incurred if sequencing does not
begin today (in 2 years; in 5 years,  in 10 years)?

5. What type of organizational model for genome sequencing would you support: 
sequencing centers vs. individual interested labs?
6. What quality standards would you expect for the sequence: high or low  
accuracy (high accuracy = higher cost)?

The committee looks forward to your comments on these important issues.


Joe Ecker (for the NAASC)
Univ. of Pennsylvania
fax (215) 898-8780

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