I am writing to ask if anyone has a method for GUS staining mature arabidopsis
seeds since it is difficult to infiltrate the x-gluc through the seed coat. I
have heard of a methanol based x-gluc substrate and I would appreciate it if
someone has the protocol to send it to me. If anyone has any suggestions or
protocols of how to stain the seeds, leaving them intact I would be grateful.
Please e-mail botrm at lure.latrobe.edu.au