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arabidopsis seeds

pstaswick at crcvms.unl.edu pstaswick at crcvms.unl.edu
Mon May 23 13:24:59 EST 1994

In article <2rirqs$rjo at mserv1.dl.ac.uk>, STEPHEN A ROLFE <S.Rolfe at sheffield.ac.uk> writes:
> Please excuse this extremely lo-tech question.  What is the best way
> to evenly disperse seeds on an agar plate for kanamycin selection?  I

A previous post doc of mine (Triant Stasinopolous) sterilized seeds in the 
normal way and then dried them on sterile filter paper in the hood. They can
be dispersed fairly evenly on plates by creasing the filter and gently tapping
the seeds off onto plates. Dried seed could also be stored for quite a while
and remained sterile if properly packaged.

Paul Staswick
Department of Agronomy
University of Nebraska

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