Similar, to a recently asked question on the network, I have had to work
with the low copy number PBI binary plasmids for some time. They don't give
me much yield from a mini prep, and I'm looking for a high copy number
binary plasmid, with a unique Eco R I site at the present time.
But more generally, are there any other binary plasmids which do have a
higher copy number than the PBI ones, with useful restriction sites.
pb23 at umail.umd.edu